The Chatterjee lab, a part of the Mycobacteria Research Laboratories, focuses on the glycomics and glycobiology of the mycobacterial cell wall glycans such as lipopolysaccharides, glycolipids, and neutral glycans – playing important roles in the physiology and/or pathogenicity of Mycobacterium tuberculosis/leprae or in non- tuberculous mycobacteria.
In early years, Dr. Chatterjee’s laboratory focused on glycomics, glycobiology, genetics and pathogenesis of lipoarabinomannan (LAM), a major cell wall component of mycobacteria, that has antigenic properties. A major portion of this work, that was funded by WHO for two years and NIH for thirteen years, contributed to our present understanding of the role of LAM in pathogenesis. This also provided the premise for investigating LAM as a biomarker for TB diagnostics.
Over the last decade, Dr. Chatterjee’s laboratory has made efforts to advance the knowledge and resources for the global mycobacteria research community and work with others in the field to continue efforts on developing a “field applicable” diagnostic platform that will have the potential to impact multiple populations and communities. This work was funded in initial years by Bill and Melinda Gates foundation and is currently being supported by NIH. The lab also extensively uses Mass Spectrometry (MS) and recently methods have been developed to analyze oligosaccharides without any derivatization. The Gas Chromatography- Mass Spectrometry (GC-MS) has also been used in the lab for the validation of TB patients or suspects from different geographical locations to ascertain the presence or absence of LAM in urine for diagnostics and quantification. The lab has also worked on the metabolomics analysis of granuloma development in the lungs of animals infected with M. tuberculosis and showed for the first time that semi solid NMR i.e; High Resolution Magic Angle Spinning (HR-MAS) can be applied directly to TB infected granulomas.
Patents & IPs
Device and Methods for Diagnosis of Active Tuberculosis
Inventors: Delphi Chatterjee et al.
CSURF Ref. No. 2021-083 (US)
Methods of Detecting Lipoarabinomannan in Urine and Diagnosing Nontuberculosis Mycobacterial Infection
(CSU) Provisional Patent 2879-224-PROV
Overcome low levels of detection limit and choice of antibody affects detection of lipoarabinomannan in pediatric tuberculosis.
Amin AG, De P, Graham B, Jensen BL, Moreau E, Chatterjee D.
PLoS One. 2022 Oct 11;17(10):e0275838. doi: 10.1371/journal.pone.0275838. eCollection 2022. PMID: 36219600
Urine lipoarabinomannan in HIV uninfected, smear negative, symptomatic TB patients: effective sample pretreatment for a sensitive immunoassay and mass spectrometry.
Amin AG, De P, Graham B, Calderon RI, Franke MF, Chatterjee D.
Sci Rep. 2021 Feb 3;11(1):2922. doi: 10.1038/s41598-021-82445-4. PMID: 33536495
Wallace E, Hendrickson D, Tolli N, Mehaffy C, Peña M, Nick JA, Knabenbaur P, Watkins J, Simpson A, Amin AG, Chatterjee D, Dobos KM, Lahiri R, Adams L, Strong M, Salfinger M, Bradford R, Stedman TT, Riojas MA, Hazbón MH.
Methods Mol Biol. 2021;2314:1-58. doi: 10.1007/978-1-0716-1460-0_1. PMID: 34235647
J Cyst Fibros. 2020 Sep;19(5):801-807. doi: 10.1016/j.jcf.2020.06.016. Epub 2020 Jul 3.PMID: 32624408
Comparative Structural Study of Terminal Ends of Lipoarabinomannan from Mice Infected Lung Tissues and Urine of a Tuberculosis Positive Patient.
ACS Infect Dis. 2020 Feb 14;6(2):291-301. doi: 10.1021/acsinfecdis.9b00355. Epub 2019 Dec 12.PMID: 31762254
news and updates
Microbiology undergraduate students Joshua Daum and Roslyn Dermody, mentored by Delphi Chatterjee, receive highest honors for their research.
Anita Amin and Prithwiraj De from the Chatterjee lab led high school students through hands-on lab activities during 2017 World TB Day at CSU.
Through an international partnership, CSU mycobacteria researchers served as guest instructors for the workshop at South Asian University.
Lab: Microbiology room C311
Office: Microbiology room C320
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