Chatterjee Lab

The Chatterjee lab, in the Mycobacteria Research Laboratories, a part of the Department of Microbiology, Immunology & Pathology, focuses on the glycomics and glycobiology of the mycobacterial cell wall glycans such as lipopolysaccharides, glycolipids, and neutral glycans – playing important roles in the physiology and/or pathogenicity of Mycobacterium tuberculosis/leprae or in non- tuberculous mycobacteria.

In early years, Dr. Chatterjee’s laboratory focused on glycomics, glycobiology, genetics and pathogenesis of lipoarabinomannan (LAM), a major cell wall component of mycobacteria, that has antigenic properties. A major portion of this work, that was funded by WHO for two years and NIH for thirteen years, contributed to our present understanding of the role of LAM in pathogenesis. This also provided the premise for investigating LAM as a biomarker for TB diagnostics.

Over the last decade, Dr. Chatterjee’s laboratory has made efforts to advance the knowledge and resources for the global mycobacteria research community and work with others in the field to continue efforts on developing a “field applicable” diagnostic platform that will have the potential to impact multiple populations and communities. This work was funded in initial years by Bill and Melinda Gates foundation and is currently being supported by NIH. The lab also extensively uses Mass Spectrometry (MS) and  recently methods have been developed to analyze oligosaccharides without any derivatization. The Gas Chromatography- Mass Spectrometry (GC-MS) has also been used in the lab for the validation of TB patients or suspects from different geographical locations to ascertain the presence or absence of LAM in urine for diagnostics and quantification. The lab has also worked on the metabolomics analysis of granuloma development in the lungs of animals infected with M. tuberculosis and showed for the first time that semi solid NMR i.e; High Resolution Magic Angle Spinning (HR-MAS) can be applied directly to TB infected granulomas.

research project

Role of Gamma/Delta T Cells in Vaccine Induced Immunity

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research project

TB Diagnostic Research: Provision and Characterization of Lipoarabinomannan from clinical isolates

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research project

Validation of urine/serum LAM in HIV/nonHIV TB suspects and POC Test Development

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research project

Urine Lipoarabinomannan as a Marker for Low-risk of NTM Airway Infection

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Publications

Urine lipoarabinomannan in HIV uninfected, smear negative, symptomatic TB patients: effective sample pretreatment for a sensitive immunoassay and mass spectrometry.

Amin AG, De P, Graham B, Calderon RI, Franke MF, Chatterjee D.
Sci Rep. 2021 Feb 3;11(1):2922. doi: 10.1038/s41598-021-82445-4. PMID: 33536495

Culturing Mycobacteria.

Wallace E, Hendrickson D, Tolli N, Mehaffy C, Peña M, Nick JA, Knabenbaur P, Watkins J, Simpson A, Amin AG, Chatterjee D, Dobos KM, Lahiri R, Adams L, Strong M, Salfinger M, Bradford R, Stedman TT, Riojas MA, Hazbón MH.
Methods Mol Biol. 2021;2314:1-58. doi: 10.1007/978-1-0716-1460-0_1. PMID: 34235647

Urine lipoarabinomannan as a marker for low-risk of NTM infection in the CF airway.

De P, Amin AG, Graham B, Martiniano SL, Caceres SM, Poch KR, Jones MC, Saavedra MT, Malcolm KC, Nick JA, Chatterjee D.
J Cyst Fibros. 2020 Sep;19(5):801-807. doi: 10.1016/j.jcf.2020.06.016. Epub 2020 Jul 3.PMID: 32624408

Comparative Structural Study of Terminal Ends of Lipoarabinomannan from Mice Infected Lung Tissues and Urine of a Tuberculosis Positive Patient.

De P, Shi L, Boot C, Ordway D, McNeil M, Chatterjee D.
ACS Infect Dis. 2020 Feb 14;6(2):291-301. doi: 10.1021/acsinfecdis.9b00355. Epub 2019 Dec 12.PMID: 31762254

Structural determinants in a glucose-containing lipopolysaccharide from Mycobacterium tuberculosis critical for inducing a subset of protective T cells.

De P, McNeil M, Xia M, Boot CM, Hesser DC, Denef K, Rithner C, Sours T, Dobos KM, Hoft D, Chatterjee D.
J Biol Chem. 2018 Jun 22;293(25):9706-9717. doi: 10.1074/jbc.RA118.002582. Epub 2018 May 1. PMID: 29716995

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People

Delphi Chatterjee, Ph.D.

Lab Principal Investigator [PI]
Professor

Anita Amin, M.S.

Research Scientist IV
Laboratory Manager

Yosita Panraksa, Ph.D.

Postdoctoral Fellow

Brooke Jensen

Student Researcher

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