Crick Lab

The Crick laboratory has pursued biochemical studies on the structure and biogenesis of the cell-wall and lipids of Mycobacterium tuberculosis since joining the Mycobacteria Research Laboratories at the Colorado State University Department of Microbiology, Immunology and Pathology.  The primary focus of the lab is in the development of enzyme assays and characterization of enzymes and metabolic pathways although we also have experience synthesizing substrates and products for enzymatic reactions and have numerous publications detailing characterization of and development of high throughput screens for many enzymes expressed in M. tuberculosis.

Cell wall synthesis

The laboratory has been among the front runners in establishing the structure and synthesis of mycobacteria cell wall.  This has resulted in numerous publications on cell wall structure and novel modifications in these pathogenic bacteria, as well as, identification and characterization of the enzymes responsible for many of these novel features.

Isopentenyl diphosphate synthesis

Isopentenyl diphosphate is as obligate intermediate in the synthesis of all isoprenoid compounds.  We have demonstrated that M. tuberculosis utilizes the methylerythritol phosphate (MEP) pathway for synthesis of isopentenyl diphosphate rather than the mevalonate pathway utilized by Homo sapiens and many other eukaryotes.  Since the MEP pathway does not exist in man it is generally thought to be a promising drug target and we have cloned, expressed, characterized and evaluated most of the enzymes in the MEP pathway as potential drug targets in M. tuberculosis and other pathogenic bacteria.

Menaquinone synthesis

The laboratory recently been involved in the study of the function and synthesis of menaquinone in mycobacteria and we were among the first proponents of the pathway as a potential drug target in M. tuberculosis.

Drug target analysis in collaboration with various groups working on diverse antibiotics

Identification of the target of new antimicrobials is critical to understanding mechanism of action as well as designing improved compounds.  We have collaborated with a number of groups that are interested in or synthesizing new antimicrobials and provided insight into the mechanism of action of a number of promising inhibitors.


  • Composition and Methods of Use of Electron Transport System Inhibitors Serial Number: PCT / US 2007 / 63122. Kurosu, M.; Crick, D. C. US Patent 2007.
  • Acid and Base Stable Diphenylmethanol Derivatives and Method of Use. Kurosu, M.; Crick, D. C. US Patent February 2008.
  • Combined Anti-Acid-Fast Bacterial Agent, Screening Method for Anti-Acid-Fast Bacterial agents, and Activity Inhibitor of WecA or Ortholog Thereof. Ishizaki, Y.; Igarashi, M.; Brennan, P.J.; Crick, D.C.  US Patent December 2017.


SAR study of piperidine derivatives as inhibitors of 1,4-dihydroxy-2-naphthoate isoprenyltransferase (MenA) from Mycobacterium tuberculosis.
Berg K, Hegde P, Pujari V, Brinkmann M, Wilkins DZ, Parish T, Crick DC, Aldrich CC.
Eur J Med Chem. 2023 Mar 5;249:115125. doi: 10.1016/j.ejmech.2023.115125. Epub 2023 Jan 18. PMID: 36682292

LdtC Is a Key l,d-Transpeptidase for Peptidoglycan Assembly in Mycobacterium smegmatis.
Francis ZK, Sanders AN, Crick DC, Mahapatra S, Islam MN, Pavelka MS Jr.
J Bacteriol. 2023 Jan 26;205(1):e0042422. doi: 10.1128/jb.00424-22. Epub 2022 Dec 21. PMID: 36541811

Mycobacterial MenG: Partial Purification, Characterization, and Inhibition.
Pujari V, Rozman K, Dhiman RK, Aldrich CC, Crick DC.
ACS Infect Dis. 2022 Dec 9;8(12):2430-2440. doi: 10.1021/acsinfecdis.2c00190. Epub 2022 Nov 23. PMID: 36417754

Do bioactive 8-hydroxyquinolines oxidovanadium(IV) and (V) complexes inhibit the growth of M. smegmatis?
Scalese G, Arhouma Z, Kostenkova K, Pérez-Díaz L, Crick DC, Gambino D, Crans DC.
J Inorg Biochem. 2022 Dec;237:111984. doi: 10.1016/j.jinorgbio.2022.111984. Epub 2022 Sep 3. PMID: 36152468

Electron Transport Lipids Fold Within Membrane-Like Interfaces.
Braasch-Turi MM, Koehn JT, Kostenkova K, Van Cleave C, Ives JW, Murakami HA, Crick DC, Crans DC.
Front Chem. 2022 Mar 8;10:827530. doi: 10.3389/fchem.2022.827530. eCollection 2022. PMID: 35350775

more publications


Dean Crick, Ph.D.

Lab Principal Investigator [PI]
Director, Mycobacteria Research Laboratories

Venugopal Pujari, Ph.D.

Research Scientist I

news and updates view all