Dobos Lab

The Dobos laboratory is focused on descriptive, comparative studies of Mycobacterium tuberculosis strains that are relevant to the development of second-generation vaccines and diagnostic assays. Specifically, the laboratory utilizes mass spectrometry, animal models of tuberculosis, and collaborations with clinical research groups to define macromolecules engaged in host-parasite interactions. This is accomplished through highly collaborative studies within CSU (principally with the laboratories of Drs. Angelo Izzo and Nicole Kruh-Garcia) and outside of the University.

research project

Microbiology and Infectious Diseases Biological Research Repository (MID-BRR)

The Dobos laboratory provides crude and purified reagents from M. tuberculosis, M. leprae, and other specialized Mycobacterium species and transfers these reagents to the MID-BRR repository managed by American Type Culture Collection. Products include irradiated cells, subcellular fractions, genomic DNA, crude lipids, purified lipids, lipoglycans, native proteins, and antibodies/antisera.

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research project

Collaborations with Oregon Health and Sciences University

The Lewinsohn lab focuses on understanding how human CD8+ T cells recognize cell infected with Mycobacterium tuberculosis (Mtb). Areas of interest include defining the repertoire of immunodominant antigens in Mtb, defining the mechanisms by which Mtb-antigens can enter the HLA-I antigen processing pathway, and defining the mechanisms by which innate T cells can recognize cells infected with Mtb.

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research project

Collaborations with Saint Louis University

Dr. Daniel Hoft treats patients for infectious diseases. His areas of expertise include tuberculosis, parasitology, and vaccination. He is particularly interested in research into vaccine development, especially for Chagas disease, pandemic influenza, and tuberculosis.

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research project

CDC PHEPRAR contract

The Dobos lab will establish conditions for kill curves for requested genus. After surrogate kill curves are established, ATCC will provide “select agent” genus/species to be tested. These will be irradiated at one dose higher than the minimum dose required to kill the surrogate. The Dobos laboratory provides scientific expertise on the irradiation of select agents and surrogates/near neighbors.

Publications

Nontuberculous Mycobacteria Show Differential Infectivity and Use Phospholipids to Antagonize LL-37.

Honda JR, Hess T, Carlson R, Kandasamy P, Nieto Ramirez LM, Norton GJ, Virdi R, Islam MN, Mehaffy C, Hasan NA, Epperson LE, Hesser D, Alper S, Strong M, Flores SC, Voelker DR, Dobos KM, Chan ED.
Am J Respir Cell Mol Biol. 2019 Sep 23. doi: 10.1165/rcmb.2018-0278OC. [Epub ahead of print]

Protein profile of different cellular fractions from Mycobacterium tuberculosis strains after exposure to isoniazid.

Nieto Ramirez LM1,2, Mehaffy C1Dobos KM1.
Data Brief. 2019 Apr 25;24:103953. doi: 10.1016/j.dib.2019.103953. eCollection 2019 Jun.

Mycobacteria and their sweet proteins: An overview of protein glycosylation and lipoglycosylation in M. tuberculosis.

Mehaffy C, Belisle JT, Dobos KM.
Tuberculosis (Edinb). 2019 Mar;115:1-13. doi: 10.1016/j.tube.2019.01.001. Epub 2019 Jan 14

Deciphering the molecular basis of mycobacteria and lipoglycan recognition by the C-type lectin Dectin-2.

Decout A, Silva-Gomes S, Drocourt D, Blattes E, Rivière M, Prandi J, Larrouy-Maumus G, Caminade AM, Hamasur B, Källenius G1, Kaur D, Dobos KM, Lucas M, Sutcliffe IC, Besra GS, Appelmelk BJ, Gilleron M, Jackson M, Vercellone A, Tiraby G, Nigou J.
Sci Rep. 2018 Nov 15;8(1):16840. doi: 10.1038/s41598-018-35393-5.

Structural determinants in a glucose-containing lipopolysaccharide from Mycobacterium tuberculosis critical for inducing a subset of protective T cells.

De P, McNeil M, Xia M, Boot CM, Hesser DC, Denef K, Rithner C, Sours T, Dobos KM, Hoft D, Chatterjee D.
J Biol Chem. 2018 Jun 22;293(25):9706-9717. doi: 10.1074/jbc.RA118.002582. Epub 2018 May 1.

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People

Karen Dobos

Lab Principal Investigator (PI)
Professor
Director, Research Integrity & Compliance Review Office (RICRO)

Carolina Mehaffy

Research Scientist/Scholar II

Megan Lucas

Laboratory Manager, BEI Project Manager

Danny Hesser

Research Associate III

Anne Simpson

Research Associate II

Kala Early

Research Assistant I

Danara Flores

Research Assistant I

Jackson Watkins

Graduate Assistant

Joseph Canterbury

Student Researcher

Madison Stoltz

Student Researcher

Nile Stoltz

Student Researcher

Madison Russell

Student Researcher

Alex Lucas

High School Student Researcher

Dobos lab retreat at the Estes Park ropes course.

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