Dobos Lab

The Dobos laboratory is focused on descriptive, comparative studies of Mycobacterium tuberculosis strains that are relevant to the development of second-generation vaccines and diagnostic assays. Specifically, the laboratory utilizes mass spectrometry, animal models of tuberculosis, and collaborations with clinical research groups to define macromolecules engaged in host-parasite interactions. This is accomplished through highly collaborative studies within CSU and outside of the University.

research project

Microbiology and Infectious Diseases Biological Research Repository (MID-BRR)

The Dobos laboratory provides crude and purified reagents from M. tuberculosis, M. leprae, and other specialized Mycobacterium species and transfers these reagents to the MID-BRR repository managed by American Type Culture Collection. Products include irradiated cells, subcellular fractions, genomic DNA, crude lipids, purified lipids, lipoglycans, native proteins, and antibodies/antisera.

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research project

Collaborations with Oregon Health and Sciences University

The Lewinsohn lab focuses on understanding how human CD8+ T cells recognize cell infected with Mycobacterium tuberculosis (Mtb). Areas of interest include defining the repertoire of immunodominant antigens in Mtb, defining the mechanisms by which Mtb-antigens can enter the HLA-I antigen processing pathway, and defining the mechanisms by which innate T cells can recognize cells infected with Mtb.

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research project

Collaborations with Saint Louis University

Dr. Daniel Hoft treats patients for infectious diseases. His areas of expertise include tuberculosis, parasitology, and vaccination. He is particularly interested in research into vaccine development, especially for Chagas disease, pandemic influenza, and tuberculosis.

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research project

CDC PHEPRAR contract

The Dobos lab will establish conditions for kill curves for requested genus. After surrogate kill curves are established, ATCC will provide “select agent” genus/species to be tested. These will be irradiated at one dose higher than the minimum dose required to kill the surrogate. The Dobos laboratory provides scientific expertise on the irradiation of select agents and surrogates/near neighbors.

Publications

Identification of Mycobacterium tuberculosis Peptides in Serum Extracellular Vesicles from Persons with Latent Tuberculosis Infection.

Mehaffy C, Kruh-Garcia NA, Graham B, Jarlsberg LG, Willyerd CE, Borisov A, Sterling TR, Nahid P, Dobos KM.
J Clin Microbiol. 2020 May 26;58(6):e00393-20. doi: 10.1128/JCM.00393-20. Print 2020 May 26. PMID: 32245831

Nontuberculous Mycobacteria Show Differential Infectivity and Use Phospholipids to Antagonize LL-37.

Honda JR, Hess T, Carlson R, Kandasamy P, Nieto Ramirez LM, Norton GJ, Virdi R, Islam MN, Mehaffy C, Hasan NA, Epperson LE, Hesser D, Alper S, Strong M, Flores SC, Voelker DR, Dobos KM, Chan ED.
Am J Respir Cell Mol Biol. 2020 Mar;62(3):354-363. doi: 10.1165/rcmb.2018-0278OC. PMID: 31545652

Protein profile of different cellular fractions from Mycobacterium tuberculosis strains after exposure to isoniazid.

Nieto Ramirez LM, Mehaffy C, Dobos KM.
Data Brief. 2019 Apr 25;24:103953. doi: 10.1016/j.dib.2019.103953. eCollection 2019 Jun.

Mycobacteria and their sweet proteins: An overview of protein glycosylation and lipoglycosylation in M. tuberculosis.

Mehaffy C, Belisle JT, Dobos KM.
Tuberculosis (Edinb). 2019 Mar;115:1-13. doi: 10.1016/j.tube.2019.01.001. Epub 2019 Jan 14

Deciphering the molecular basis of mycobacteria and lipoglycan recognition by the C-type lectin Dectin-2.

Decout A, Silva-Gomes S, Drocourt D, Blattes E, Rivière M, Prandi J, Larrouy-Maumus G, Caminade AM, Hamasur B, Källenius G1, Kaur D, Dobos KM, Lucas M, Sutcliffe IC, Besra GS, Appelmelk BJ, Gilleron M, Jackson M, Vercellone A, Tiraby G, Nigou J.
Sci Rep. 2018 Nov 15;8(1):16840. doi: 10.1038/s41598-018-35393-5.

more publications

People

Karen Dobos, Ph.D.

Lab Principal Investigator (PI)
Professor
Scientific Director, CSU Regional Biocontainment Laboratory
Co-Director, Mycobacteria Research Laboratories

Carolina Mehaffy, Ph.D.

Assistant Professor

Megan Lucas

Laboratory and BEI Project Manager
Research Associate IV

Anne Simpson

Research Associate III

Rebecca Staudenmaier

Research Associate II

Kieran Broder

Research Associate I

Vinnie Guglielmi

Research Associate I

Briana Marsico

Research Associate I

Megan Stookey

Research Associate I

Nurudeen Oketade

Graduate Research Assistant

Kimberly Shelton

Graduate Research Assistant

Dayton McGrail

Student Researcher

Jonah Davis

Student Researcher

Peyton Slack

Coordinator

Dobos lab retreat at the Estes Park ropes course (2019).

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