The Dobos laboratory is focused on descriptive, comparative studies of Mycobacterium tuberculosis strains that are relevant to the development of second-generation vaccines and diagnostic assays. Specifically, the laboratory utilizes mass spectrometry, animal models of tuberculosis, and collaborations with clinical research groups to define macromolecules engaged in host-parasite interactions. This is accomplished through highly collaborative studies within CSU and outside of the University.
The Dobos lab has been certified as a Green Level Certified Lab as of February 9, 2023. Learn more about My Green Lab certification or visit the CSU Green Labs website.
research project
Microbiology and Infectious Diseases Biological Research Repository (MID-BRR)
The Dobos laboratory provides crude and purified reagents from M. tuberculosis, M. leprae, and other specialized Mycobacterium species and transfers these reagents to the MID-BRR repository managed by American Type Culture Collection. Products include irradiated cells, subcellular fractions, genomic DNA, crude lipids, purified lipids, lipoglycans, native proteins, and antibodies/antisera.
view project
research project
Collaborations with Oregon Health and Sciences University
The Lewinsohn lab focuses on understanding how human CD8+ T cells recognize cell infected with Mycobacterium tuberculosis (Mtb). Areas of interest include defining the repertoire of immunodominant antigens in Mtb, defining the mechanisms by which Mtb-antigens can enter the HLA-I antigen processing pathway, and defining the mechanisms by which innate T cells can recognize cells infected with Mtb.
view project
research project
Collaborations with Saint Louis University
Dr. Daniel Hoft treats patients for infectious diseases. His areas of expertise include tuberculosis, parasitology, and vaccination. He is particularly interested in research into vaccine development, especially for Chagas disease, pandemic influenza, and tuberculosis.
view project
research project
CDC PHEPRAR contract
The Dobos lab will establish conditions for kill curves for requested genus. After surrogate kill curves are established, ATCC will provide “select agent” genus/species to be tested. These will be irradiated at one dose higher than the minimum dose required to kill the surrogate. The Dobos laboratory provides scientific expertise on the irradiation of select agents and surrogates/near neighbors.
Publications
Mehaffy C, Kruh-Garcia NA, Graham B, Jarlsberg LG, Willyerd CE, Borisov A, Sterling TR, Nahid P, Dobos KM.
J Clin Microbiol. 2020 May 26;58(6):e00393-20. doi: 10.1128/JCM.00393-20. Print 2020 May 26. PMID: 32245831
Honda JR, Hess T, Carlson R, Kandasamy P, Nieto Ramirez LM, Norton GJ, Virdi R, Islam MN, Mehaffy C, Hasan NA, Epperson LE, Hesser D, Alper S, Strong M, Flores SC, Voelker DR, Dobos KM, Chan ED.
Am J Respir Cell Mol Biol. 2020 Mar;62(3):354-363. doi: 10.1165/rcmb.2018-0278OC. PMID: 31545652
Nieto Ramirez LM, Mehaffy C, Dobos KM.
Data Brief. 2019 Apr 25;24:103953. doi: 10.1016/j.dib.2019.103953. eCollection 2019 Jun.
Mehaffy C, Belisle JT, Dobos KM.
Tuberculosis (Edinb). 2019 Mar;115:1-13. doi: 10.1016/j.tube.2019.01.001. Epub 2019 Jan 14
Decout A, Silva-Gomes S, Drocourt D, Blattes E, Rivière M, Prandi J, Larrouy-Maumus G, Caminade AM, Hamasur B, Källenius G1, Kaur D, Dobos KM, Lucas M, Sutcliffe IC, Besra GS, Appelmelk BJ, Gilleron M, Jackson M, Vercellone A, Tiraby G, Nigou J.
Sci Rep. 2018 Nov 15;8(1):16840. doi: 10.1038/s41598-018-35393-5.
more publications
People
Karen Dobos, Ph.D.
Lab Principal Investigator (PI)
Professor
Scientific Director, CSU Regional Biocontainment Laboratory
Co-Director, Mycobacteria Research Laboratories
Megan Lucas
Laboratory and BEI Project Manager
Research Associate IV
Dobos lab retreat at the Estes Park ropes course (2019).