Furthermore, as the Director of the CSU Flow Cytometry Core facility and due to our labs experience using flow cytometry as an analytical tool, our laboratory has been implementing and developing new tools for multicolor flow cytometry analysis. We are currently using algorithms that automatically identify cell populations according to their marker expression profiles. These computational methods not only have the capacity to identify rare populations, but also to match cell populations across samples, and statistically compare features between different populations. These algorithms provide an unsupervised analysis, allowing an unbiased investigation of cytometry data. In addition to flow cytometry, we are currently implementing single cell sequencing, as well as, metabolomic analysis of immune cells elicited in response to mycobacterial infections and vaccination
Immune Mechanisms of Protection Against Mycobacterium tuberculosis Center (IMPAC-TB)
The Henao Tamayo and Podell labs are leading a CSU team on a $1.2 million subcontract to accelerate research progress in tuberculosis vaccines. Objectives are to understand the immune responses that prevents initial infection, the establishment of latent infection, and the transition to active TB disease. There is currently only one vaccine for TB and it only reliably works on children.
Vaccine induced memory immunity in tuberculosis
There are several vaccine candidates that give protection against the laboratory strains H37Rv and Erdman at a level comparable to the BCG vaccine. However, whether different vaccine types give equivalent or different levels of memory T cell subsets is unknown, and whether these vaccines will be equally protective against newly emerging highly virulent clinical strains is equally unaddressed.view project
Cyto-Feature Engineering: A Pipeline for Flow Cytometry Analysis to Uncover Immune Populations and Associations with Disease
Amy Fox, Taru S. Dutt, Burton Karger, Mauricio Rojas, Andrés Obregón-Henao, G. Brooke Anderson, Marcela Henao-Tamayo
Scientific Reports volume 10, Article number: 7651 (2020)
Trying to See the Forest through the Trees: Deciphering the Nature of Memory Immunity to Mycobacterium tuberculosis.
Orme IM, Henao-Tamayo MI.
Front Immunol. 2018 Mar 8;9:461. doi: 10.3389/fimmu.2018.00461. eCollection 2018. Review.
Ramos L, Obregon-Henao A, Henao-Tamayo M, Bowen R, Izzo A, Lunney JK, Gonzalez-Juarrero M.
Vet Immunol Immunopathol. 2019 Sep;215:109884. doi: 10.1016/j.vetimm.2019.109884. Epub 2019 Jul 26.
Shanley CA, Henao-Tamayo MI, Bipin C, Mugasimangalam R, Verma D, Ordway DJ, Streicher EM, Orme IM.
Tuberculosis (Edinb). 2018 Mar;109:123-133. doi: 10.1016/j.tube.2018.02.003. Epub 2018 Feb 9.
King TH, Shanley CA, Guo Z, Bellgrau D, Rodell T, Furney S, Henao-Tamayo M, Orme IM.
Clin Vaccine Immunol. 2017 Dec 5;24(12). pii: e00245-17. doi: 10.1128/CVI.00245-17. Print 2017 Dec.
Research Scientist III
Research Associate II, Lab Manager
CSU-Flow Cytometry Core Manager
news and updates
Drs. Henao-Tamayo and Podell received an award to work on tuberculosis vaccines.
Dr. Marcela Heano-Tamayo leads the Flow Cytometry & Cell Sorting Core.
Dr. Henao-Tamayo speaks about her lab’s use of spectral flow cytometry to study tuberculosis.
Microbiology room C210