Furthermore, as the Director of the CSU Flow Cytometry Core facility and due to our labs experience using flow cytometry as an analytical tool, our laboratory has been implementing and developing new tools for multicolor flow cytometry analysis. We are currently using algorithms that automatically identify cell populations according to their marker expression profiles. These computational methods not only have the capacity to identify rare populations, but also to match cell populations across samples, and statistically compare features between different populations. These algorithms provide an unsupervised analysis, allowing an unbiased investigation of cytometry data. In addition to flow cytometry, we are currently implementing single cell sequencing, as well as, metabolomic analysis of immune cells elicited in response to mycobacterial infections and vaccination
Immune Mechanisms of Protection Against Mycobacterium tuberculosis Center (IMPAC-TB)
The Henao Tamayo lab, in collaboration with the Podell lab, is leading a CSU research team on a $1.2 million subcontract from the Infectious Disease Research Center (IDRI) in Seattle. The subcontract is part of one of three centers, funded by the National Institute of Allergy and Infectious Diseases (NIAID), to accelerate immunology research progress in tuberculosis vaccine development. The overall objective is to develop a comprehensive understanding of the immune responses required to prevent initial infection, the establishment of latent infection, and the transition to active TB disease. Additionally, CSU will conduct experiments to study the interference of environmental mycobacteria, which live in water and soil, with the protection that the BCG vaccine provides against TB. There is currently only one vaccine for TB, and it only reliably works on children; however, the ability of this vaccine to protect adults is highly variable.
Vaccine induced memory immunity in tuberculosis
There are several vaccine candidates in development that give protection against the laboratory strains H37Rv and Erdman at a level comparable to the BCG vaccine. However, whether [a] different vaccine types give equivalent or different levels of memory T cell subsets is unknown, and [b] whether these vaccines will be equally protective against newly emerging highly virulent clinical strains is equally unaddressed. The purpose of this study is to begin to answer these questions. The lab determines if memory immunity induced by each vaccine is equally effective against four clinical isolates of Mycobacterium tuberculosis which are all highly virulent but which differ in terms of their fitness [inhibition by BCG] and in their ability to induce unbeneficial regulatory T cell responses.view project
Trying to See the Forest through the Trees: Deciphering the Nature of Memory Immunity to Mycobacterium tuberculosis.
Orme IM, Henao-Tamayo MI.
Front Immunol. 2018 Mar 8;9:461. doi: 10.3389/fimmu.2018.00461. eCollection 2018. Review.
Ramos L, Obregon-Henao A, Henao-Tamayo M, Bowen R, Izzo A, Lunney JK, Gonzalez-Juarrero M.
Vet Immunol Immunopathol. 2019 Sep;215:109884. doi: 10.1016/j.vetimm.2019.109884. Epub 2019 Jul 26.
Shanley CA, Henao-Tamayo MI, Bipin C, Mugasimangalam R, Verma D, Ordway DJ, Streicher EM, Orme IM.
Tuberculosis (Edinb). 2018 Mar;109:123-133. doi: 10.1016/j.tube.2018.02.003. Epub 2018 Feb 9.
King TH, Shanley CA, Guo Z, Bellgrau D, Rodell T, Furney S, Henao-Tamayo M, Orme IM.
Clin Vaccine Immunol. 2017 Dec 5;24(12). pii: e00245-17. doi: 10.1128/CVI.00245-17. Print 2017 Dec.
The minipig as an animal model to study Mycobacterium tuberculosis infection and natural transmission.
Ramos L, Obregon-Henao A, Henao-Tamayo M, Bowen R, Lunney JK, Gonzalez-Juarrero M.
Tuberculosis (Edinb). 2017 Sep;106:91-98. doi: 10.1016/j.tube.2017.07.003. Epub 2017 Jul 14.
Research Scientist III
CSU-Flow Cytometry Core Coordinator
Research Associate II, Lab Manager
news and updates
Read about the awarded tuberculosis vaccine research led by Drs. Henao Tamayo & Podell.
Learn about the Colorado State University Flow Cytometry & Cell Sorting Core.
Watch Dr. Henao-Tamayo speak about her lab’s use of spectral flow cytometry to study tuberculosis.
Microbiology room C210