Henao Tamayo Lab
Our laboratory is devoted to investigate the pathogenesis and immune response to mycobacterial species, specially M. tuberculosis, with a central interest in the adaptive immune response induced by vaccines. In our laboratory we have the ability to test how effective this immune response is against different clinical strains of tuberculosis. Our goal is to achieve a better understanding of the immune response to different vaccine candidates using a multiparametric, unbiased analysis of the cells induced after vaccination. We also utilize metabolic profiles and single cell sequencing as a readout of the type of cellular immune response induced after vaccination.

Furthermore, as the Director of the CSU Flow Cytometry Core facility and due to our labs experience using flow cytometry as an analytical tool, our laboratory has been implementing and developing new tools for multicolor flow cytometry analysis. We are currently using algorithms that automatically identify cell populations according to their marker expression profiles. These computational methods not only have the capacity to identify rare populations, but also to match cell populations across samples, and statistically compare features between different populations. These algorithms provide an unsupervised analysis, allowing an unbiased investigation of cytometry data. In addition to flow cytometry, we are currently implementing single cell sequencing, as well as, metabolomic analysis of immune cells elicited in response to mycobacterial infections and vaccination
research project

Immune Mechanisms of Protection Against Mycobacterium tuberculosis Center (IMPAC-TB)

The Henao Tamayo and Podell labs are leading a CSU team on a $1.2 million subcontract to accelerate research progress in tuberculosis vaccines. Objectives are to understand the immune responses that prevents initial infection, the establishment of latent infection, and the transition to active TB disease. There is currently only one vaccine for TB and it only reliably works on children.

research project

Vaccine induced memory immunity in tuberculosis

There are several vaccine candidates that give protection against the laboratory strains H37Rv and Erdman at a level comparable to the BCG vaccine. However, whether different vaccine types give equivalent or different levels of memory T cell subsets is unknown, and whether these vaccines will be equally protective against newly emerging highly virulent clinical strains is equally unaddressed.

view project

Publications

Cyto-Feature Engineering: A Pipeline for Flow Cytometry Analysis to Uncover Immune Populations and Associations with Disease

Amy Fox, Taru S. Dutt, Burton Karger, Mauricio Rojas, Andrés Obregón-Henao, G. Brooke Anderson, Marcela Henao-Tamayo
Scientific Reports volume 10, Article number: 7651 (2020)

Trying to See the Forest through the Trees: Deciphering the Nature of Memory Immunity to Mycobacterium tuberculosis.

Orme IM, Henao-Tamayo MI.
Front Immunol. 2018 Mar 8;9:461. doi: 10.3389/fimmu.2018.00461. eCollection 2018. Review.

Minipigs as a neonatal animal model for tuberculosis vaccine efficacy testing.

Ramos L, Obregon-Henao A, Henao-Tamayo M, Bowen R, Izzo A, Lunney JK, Gonzalez-Juarrero M.
Vet Immunol Immunopathol. 2019 Sep;215:109884. doi: 10.1016/j.vetimm.2019.109884. Epub 2019 Jul 26.

Biology of clinical strains of Mycobacterium tuberculosis with varying levels of transmission.

Shanley CA, Henao-Tamayo MI, Bipin C, Mugasimangalam R, Verma D, Ordway DJ, Streicher EM, Orme IM.
Tuberculosis (Edinb). 2018 Mar;109:123-133. doi: 10.1016/j.tube.2018.02.003. Epub 2018 Feb 9.

GI-19007, a Novel Saccharomyces cerevisiae-Based Therapeutic Vaccine against Tuberculosis.

King TH, Shanley CA, Guo Z, Bellgrau D, Rodell T, Furney S, Henao-Tamayo M, Orme IM.
Clin Vaccine Immunol. 2017 Dec 5;24(12). pii: e00245-17. doi: 10.1128/CVI.00245-17. Print 2017 Dec.

more publications

People

Marcela Henao Tamayo, M.D., Ph.D.

Lab Principal Investigator (PI)
Assistant Professor

Andres Obregon Henao, Ph.D.

Research Scientist III

Burton Karger, M.S.

Research Associate II, Lab Manager

Christopher Allen, Ph.D.

CSU-Flow Cytometry Core Manager

Taru Shikha Dutt, Ph.D.

Postdoctoral Fellow

Amy Fox

Graduate Assistant

Chiara Bowen

Coordinator

Nathan Youssef

Student Researcher

news and updates view all