Engineering resistance to Zika virus in Aedes aegypti for Cas9 driven population modification
Zika is a mosquito-borne virus. Aedes aegypti is the primary mosquito vector of ZIKV, DENV and CHIKV and is found throughout the Americas. We propose to generate genetically modified Ae. aegypti that stably express anti-viral transgenes and CRISPR/Cas9 drive to efficiently spread ZIKV-refractory phenotypes in caged populations as a prelude to spreading virus resistance in field populations.
Control of RNA viruses in mosquito cells through the acquisition of vDNA and endogenous viral elements.
Tassetto M, Kunitomi M, Whitfield ZJ, Dolan PT, Sánchez-Vargas I, Garcia-Knight M, Ribiero I, Chen T, Olson KE, Andino R.
Elife. 2019 Oct 17;8. pii: e41244. doi: 10.7554/eLife.41244.
Infection with mosquito-borne alphavirus induces selective loss of dopaminergic neurons, neuroinflammation and widespread protein aggregation.
Bantle CM, Phillips AT, Smeyne RJ, Rocha SM, Olson KE, Tjalkens RB.
NPJ Parkinsons Dis. 2019 Sep 13;5:20. doi: 10.1038/s41531-019-0090-8. eCollection 2019.
The Widespread Occurrence and Potential Biological Roles of Endogenous Viral Elements in Insect Genomes.
Blair CD, Olson KE, Bonizzoni M.
Curr Issues Mol Biol. 2019 Jun 6;34:13-30. doi: 10.21775/cimb.034.013. [Epub ahead of print]
Induction of RNA interference to block Zika virus replication and transmission in the mosquito Aedes aegypti.
Magalhaes T, Bergren NA, Bennett SL, Borland EM, Hartman DA, Lymperopoulos K, Sayre R, Borlee BR, Campbell CL, Foy BD, Olson KE, Blair CD, Black W 4th, Kading RC.
Insect Biochem Mol Biol. 2019 Aug;111:103169. doi: 10.1016/j.ibmb.2019.05.004. Epub 2019 May 17.
Analysis of Salivary Glands and Saliva from Aedes albopictus and Aedes aegypti Infected with Chikungunya Viruses.
Sanchez-Vargas I, Harrington LC, Black WC 4th, Olson KE.
Insects. 2019 Feb 1;10(2). pii: E39. doi: 10.3390/insects10020039.
Arthropod-Borne and Infectious Diseases Laboratory (AIDL) room 103
AIDL room 118, Phase III BSL3 Virology Suite, Discovery Suite Insectary