The Tjalkens Laboratory investigates neuroinflammation and the role of glial cells in neurodegenerative diseases such as Parkinson’s Disease. Projects are investigating the molecular regulation of innate immune inflammatory responses in brain glial cells, as well as the impact of exposure to environmental neurotoxins on the etiology of neurodegenerative disease. Additionally, we are examining how infection with neurotropic viruses triggers inflammation and protein aggregation in neurodegenerative disorders. Transgenic models and high content fluorescence imaging are central techniques used in our laboratory to study these phenomena.
Neuroinflammation in Parkinson's Disease
Innate immune signaling in glial cells results in production of neurotoxic inflammatory factors that are associated with neuronal injury and protein aggregation in Parkinson’s disease. We are using transgenic animal models and primary cultures of microglia and astrocytes to understand the molecular pathways regulating this inflammatory phenotype.
Neurotoxicity of Heavy Metals
Neurotoxic levels of heavy metals such as manganese correlate with cognitive and behavioral impairment in both adults and children. This increases the risk for disorders such as Parkinson’s disease. Using transgenic approaches to inhibit inflammatory signaling in glial cells, we are studying the role of neuroinflammation in responses to neurotoxic heavy metals.view project
Viral Models of Neurodegeneration
Viral infection can affect the central nervous system, increasing the risk for neurological disease. Projects are exploring environmental factors modulating the capacity of selected viruses to cause inflammatory injury to the brain, including such agents as H1N1 influenza, Western Equine Encephalitis Virus and SARS-CoV-2.view project
Molecular Regulation of Neuroinflammation
Studies are examining the molecular pharmacology of small molecule regulators of inflammation in glial cells that interact with nuclear receptors to inhibit inflammatory signaling through the NF-κB pathway.
NF-κB Signaling in Astrocytes Modulates Brain Inflammation and Neuronal Injury Following Sequential Exposure to Manganese and MPTP During Development and Aging.
Toxicol Sci. 2020 Oct 1;177(2):506-520. doi: 10.1093/toxsci/kfaa115. PMID: 32692843
Infection with mosquito-borne alphavirus induces selective loss of dopaminergic neurons, inflammation and widespread protein aggregation.
NPJ Parkinsons Dis. 2019 Sep 13;5:20. doi: 10.1038/s41531-019-0090-8. eCollection 2019.
The Nurr1 Ligand,1,1-bis(3′-Indolyl)-1-(p-Chlorophenyl)Methane, Modulates Glial Reactivity and Is Neuroprotective in MPTP-Induced Parkinsonism.
J Pharmacol Exp Ther. 2018 Jun;365(3):636-651. doi: 10.1124/jpet.117.246389. Epub 2018 Apr 6.
Lab members at Canvas Stadium in 2018
Lab members hiking Horsetooth Rock Trail in August of 2019
Dr. Tjalkens with graduate student Savannah Rocha at the 2019 Mountain West Society of Toxicology Conference hosted by CSU chapter
Dr Tjalkens and Casey in BSL3 training
news and updates
Novel compound reduces viral entry and inflammation in hamsters exposed to SARS-CoV-2 (bioRxiv, 2021).
NF-kB signaling in astrocytes following exposure to Mn and MPTP (Toxicol Sci., 2020).
Juvenile exposure to Mn heightens innate immune inflammation during influenza infection (PLoS One, 2021).