Dr. John Belisle’s research focuses on the biochemistry of bacterial pathogens (in particular Mycobacterium tuberculosis, Mycobacterium leprae and Borrelia burgdorferi) and the diseases they cause: tuberculosis, leprosy and Lyme disease. He has a particular interest in how disease-causing bacteria and their hosts respond to one another at a biochemical level.
Research activities in Dr. Belisle’s laboratory have included the purification and characterization of bacterial proteins (including enzymes), lipids, and glycolconjugates; elucidation of biochemical processes; discovery of diagnostic and vaccine antigens; and the definition of specific molecular interactions involving the innate immune response. Most recently, Dr. Belisle has investigated host metabolic responses to identify biochemical pathways altered in response to bacterial infection and disease, and to apply this approach to identify novel biomarkers and biosignatures of disease progression and treatment outcome.
Dr. Belisle is co-director of the Center for Metabolism of Infectious Diseases.
The Study of Host-Pathogen Interactions in Leprosy
The pathogen Mycobacterium leprae is responsible for ~200,000 new cases of leprosy annually. The Belisle laboratory is studying how the metabolism of this obligate intracellular pathogen and the human host are entwined and drive the neuropathology of leprosy. The laboratory also studies how M. leprae products stimulate immune responses.view project
Metabolic Responses to Tuberculosis and Tuberculosis Treatment
Mycobacterium tuberculosis infections are responsible for one of the globe’s most common infectious diseases, tuberculosis. Through the application of metabolomics and other research tools, the Belisle laboratory is studying the metabolic response in tuberculosis to develop prognostic markers of disease progression and treatment response, and to further understanding of this pathogen's success.view project
Host Metabolic Biosignatures for the Diagnosis and Prognosis of Lyme Disease
Dr. Belisle’s laboratory is investigating the use of metabolomics and host metabolic biosignatures to develop novel diagnostics and prognostics tools for Lyme disease. Lyme disease, caused by Borrelia burgdorferi, is the most common vector-borne disease in the United States. Diagnosis of early Lyme disease remains difficult and delay in treatment can result in Post-Treatment Lyme Disease Syndrome.view project
The Center for Metabolism of Infectious Diseases
Along with Dr. Rushika Perera, Dr. Belisle is the co-director of the Center for Metabolism of Infectious Diseases. The center brings together the expertise of over 15 CSU research laboratories to enable development of new treatments, preventions, and diagnostics for infectious diseases by resolving host-vector-pathogen-environment interactions at a metabolic level.view project
Fitzgerald BL, Graham B, Delorey MJ, Pegalajar-Jurado A, Islam MN, Wormser GP, Aucott JN, Rebman AW, Soloski MJ, Belisle JT, Molins CR.
Clin Infect Dis. 2020 Sep 25:ciaa1455. doi: 10.1093/cid/ciaa1455.
Koh C, Islam MN, Ye YH, Chotiwan N, Graham B, Belisle JT, Kouremenos KA, Dayalan S, Tull DL, Klatt S, Perera R, McGraw EA.
Commun Biol. 2020 Sep 18;3(1):518. doi: 10.1038/s42003-020-01254-z.
Fitzgerald BL, Molins CR, Islam MN, Graham B, Hove PR, Wormser GP, Hu L, Ashton LV, Belisle JT.
J Proteome Res. 2020 Feb 7;19(2):610-623. doi: 10.1021/acs.jproteome.9b00470. Epub 2020 Jan 9.
Harris EK, Harton MR, de Mello Marques MA, Belisle JT, Molins CR, Breuner N, Wormser GP, Gilmore RD.
Sci Rep. 2019 Nov 14;9(1):16808. doi: 10.1038/s41598-019-53248-5.
Increased serum sialic acid is associated with morbidity and mortality in a murine model of dengue disease.
Espinosa DA, Beatty PR, Puerta-Guardo H, Islam MN, Belisle JT, Perera R, Harris E.
J Gen Virol. 2019 Nov;100(11):1515-1522. doi: 10.1099/jgv.0.001319.
Lab Principal Investigator
Co-Director, Center for Metabolism of Infectious Diseases (C4MInD.org)
Research Scientist II
Research Scientist I
Research Associate IV
Research Associate I
Research Associate I
news and updates
The Center for Metabolism of Infectious Diseases (C4MInD) co-led by John Belisle and Rushika Perera, enables the development of new treatments, preventions, and diagnostics for infectious diseases by resolving host-vector-pathogen-environment interactions at a metabolic level.
Awarded a 2018 Colorado Governor’s Award for High-Impact Research, CDC & CSU principal investigators explain their proof-of-concept study that can be applied to develop an innovative diagnostic test for early Lyme disease.
A team of researchers from the Centers for Disease Control and Prevention and Colorado State University was recognized with one of the 2018 CO-LABS Governor’s Awards for High-Impact Research for identifying a way to distinguish Lyme disease from similar illnesses.
Office: Research Innovation Center room D111
Lab: Research Innovation Center room D139