Gonzalez-Juarrero Lab

The Gonzalez-Juarrero laboratory focuses on the immune pathogenesis and development of aerosol therapies for mycobacterial diseases with primary emphasis on tuberculosis (TB) and non-tuberculous (NTM) diseases. Specifically, we want to understand how to enhance the antimicrobial activity in the infected host and characterize how these therapies affect the immunopathology of the disease, with an end goal of improved treatment therapies against multidrug-resistant infections.

We have developed several experimental animal models that allows for testing of aerosolized anti-TB and anti-NTM drugs. We test for efficacy, toxicity and pharmacokinetics of immunomodulators/antibiotics and study the temporal and spatial relationships of immune biomarkers during the stages of disease and therapy. Studies are done by a team of research scientists in close collaboration with other scientists in the mycobacteria research laboratories and serve to build a new generation of scientists by training graduate and undergraduate students.

Publications

Neonatal and infant immunity for tuberculosis vaccine development: importance of age-matched animal models

Laylaa RamosJoan K. LunneyMercedes Gonzalez-Juarrero

Inhaled tigecycline is effective against Mycobacterium abscessus in vitro and in vivo.

Pearce C, Ruth MM, Pennings LJ, Wertheim HFL, Walz A, Hoefsloot W, Ruesen C, Muñoz Gutiérrez J, Gonzalez-Juarrero M, van Ingen J.
J Antimicrob Chemother. 2020 Jul 1;75(7):1889-1894. doi: 10.1093/jac/dkaa110.

Development and Characterization of a Dry Powder Formulation for Anti-Tuberculosis Drug Spectinamide 1599.

Stewart IE, Lukka PB, Liu J, Meibohm B, Gonzalez-Juarrero M, Braunstein MS, Lee RE, Hickey AJ.
Pharm Res. 2019 Jul 18;36(9):136. doi: 10.1007/s11095-019-2666-8.

Comparative pharmacokinetics of spectinamide 1599 after subcutaneous and intrapulmonary aerosol administration in mice.

Rathi C, Lukka PB, Wagh S, Lee RE, Lenaerts AJ, Braunstein M, Hickey A, Gonzalez-Juarrero M, Meibohm B.
Tuberculosis (Edinb). 2019 Jan;114:119-122. doi: 10.1016/j.tube.2018.12.006. Epub 2018 Dec 31.

Host Directed Therapy for Chronic Tuberculosis via Intrapulmonary Delivery of Aerosolized Peptide Inhibitors Targeting the IL-10-STAT3 Pathway.

Upadhyay R, Sanchez-Hidalgo A, Wilusz CJ, Lenaerts AJ, Arab J, Yeh J, Stefanisko K, Tarasova NI, Gonzalez-Juarrero M.
Sci Rep. 2018 Nov 9;8(1):16610. doi: 10.1038/s41598-018-35023-0.

more publications

People

Mercedes Gonzalez-Juarrero, Ph.D.

Lab Principal Investigator [PI]
Professor

Amanda Walz, M.S.

Research Associate III

Camron Pearce, M.S.

Research Associate II
PhD Candidate

Kavita De, Ph.D.

Postdoctoral Fellow

Zohaib Ali headshot.
Zohaib Ali

PhD Candidate
Fulbright Scholar

Tucker Booth

Student Researcher

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Emma Fike

Student Researcher

Sydney Fugate

Student Researcher

Abigail Godelfer

Student Researcher

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Brandon Goral

Student Researcher

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Diane Jimenez

Student Researcher

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Ha Lam

Student Researcher

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Hunter Leyendecker

Student Researcher

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Jenna Randall

Student Researcher

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Brennan Troyer

Student Researcher

Image of foamy macrophages from the granuloma of a mouse lung infected with Mtb. The lipids are fluorescent red, the bacilli green, and the cell nuclei are stained blue.

Immunohistochemistry showing IL-10 expression (brown) in Mtb infected lung tissue.

Multiplex immunohistochemistry for different cellular markers in and around a necrotic granuloma.

Multiplex immunohistochemistry in the lung tissue section from a balb/c mouse with Mtb. Each unique color is indicative of a unique cell type.