Tesfaye Lab

The Tesfaye Laboratory investigates the molecular mechanisms associated with various aspects of female reproduction including ovarian functionality and preimplantation embryo development, predominantly using the bovine model with translational facets to humans.

The mission of the Tesfaye Lab is to:

  • Provide state-of-the-art professional training to undergraduate, graduate, and post-doctoral fellows in molecular reproductive physiology, with a special emphasis on designing experiments, interpreting data, and superior presentation and publication of results.
  • Provide trainees with the latest advancements in scientific investigation and methodologies in the field of reproductive biology.
  • Facilitate a productive and collaborative interaction between science and implementation in the field.
  • Contribute to the well-being of humans, animals, and the environment.
  • Provide a creative, supportive, collaborative, challenging, and enjoyable environment for scientific research in the field of reproductive physiology, with a special emphasis on female reproductive function.
research project

Extracellular Vesicle-coupled miRNAs in Stress Adaptation

Our lab is currently investigating the mechanisms underlying the packaging and release of precise miRNA cargo into EVs and their potential use to offset the detrimental effects of environmental stressors that ultimately affect granulosa cell function and oocyte/ embryonic development.

research project

Oviductal Organoids in Assisted Reproductive Technologies

Organoids represent biomimetic, three-dimensional, in vitro cell clusters that organizationally self-regenerate into complex structures to mirror the physiological function of their tissue of origin. Our lab currently utilizes organoids to study and recapitulate oviductal physiology in vitro, with the potential to farm EVs for downstream use in current IVF systems.

research project

Deciphering the impact of Advanced Maternal Age on Fertility

A focus in our lab is to understand the cellular and extracellular molecular responses of ovarian follicular cells and their corresponding EVs to advanced maternal age (AMA) and to investigate the possibility of developing strategies to tackle AMA-associated infertility problems.

research project

Nrf2-mediated Oxidative Stress Response

In this project, we are aiming to investigate the impact of modulating Nrf2-Keap1 signaling in bovine embryos, primarily through pharmacological (antioxidant activation) and genetic (CRISPR-Cas9 mediated knockout of Keap1) approaches on subsequent embryo survival and viability under suboptimal conditions.

research project

Antioxidant Roles in Follicular Cells and Oocytes

To improve the in vitro culture environment, our lab investigates the impact of various antioxidants (quercetin, carnosol, sulforaphane, etc.) to counteract the detrimental undertakings caused as a result of exposure to both oxidative and/or environmental stressors.

Publications

Nico G. Menjivar​, Camila Azzolin de Souza​, Ahmed Gad, Luca A. Souza, Samuel Gebremedhn, Juliano C. da Silveira, Dawit Tesfaye. 2025. Strategies for Loading miRNA Cargo into Extracellular Vesicles. Book Chapter In Neuro-methods Series (In press).

Nico G Menjivar, Ahmed Gad, Riley E Thompson, Mindy A Meyers, Soham Ghosh, Fiona K Hollinshead, Dawit Tesfaye. 2025. Organoids simulating the bovine oviduct mediate the embryo-maternal interface via extracellular vesicle-transmitted signaling, Human Reproduction Open.

Heather M Rogers, Ahmed Gad, Gentry K Cork, Nico G Menjivar, William B Schoolcraft, Dawit Tesfaye, Ye Yuan 2025. Age-related integrative transcriptomic profiling of human granulosa cells reveals mRNA-microRNA regulatory network associated with key ovulation dynamics. Biology of Reproduction.

Dessie Salilew-Wondim, Ernst Tholen, Christine Große-Brinkhaus, Eva Held-Hoelker, Dennis Miskel, Franca Rings, Karl Schellander, Urban Besenfelder, Vitezslav Havlicek, Dawit Tesfaye & Michael Hoelker. 2025. Sexually dimorphic gene expression responses of bovine embryos to the maternal microenvironment on day 13 of gestation. BMC Genomics.

Menjivar NG, Oropallo J, Gebremedhn S, Souza LA, Gad A, Puttlitz CM, Tesfaye D. 2024. MicroRNA Nano-Shuttles: Engineering Extracellular Vesicles as a Cutting-Edge Biotechnology Platform for Clinical Use in Therapeutics. Biol Proced Online.

more publications
27 undergraduate and graduate students mentored
180+ manuscripts published in scientific journals since 2003
16 grants and awards totaling over $2.4 million in funding

People

Portrait of Dawit Tesfaye
Dawit Tesfaye, Ph.D.

Principal Investigator and Associate Professor
Department of Biomedical Sciences

Ghyslaine Ramírez, D.V.M.

Ph.D. Candidate
Funding: Fulbright-CONICYT

Heather Rogers, M.S.

Ph.D. Student
Funding: Colorado Center for Reproductive Medicine

Headshot of Emilio Gutierrez
Emilio Gutierrez Castillo, Ph.D.

Postdoctoral Fellow
Department of Biomedical Sciences

Tesfaye Lab, Fall 2023

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contact information

Animal Reproduction and Biotechnology Laboratory Building, Foothills Campus
3105 Rampart Rd, Fort Collins, CO 80521
Office: W131
Laboratory: E119

(530) 564-2806