The Tesfaye Laboratory investigates the molecular mechanisms associated with various aspects of female reproduction including ovarian functionality and preimplantation embryo development, predominantly using the bovine model with translational facets to humans.
The mission of the Tesfaye Lab is to:
- Provide state-of-the-art professional training to undergraduate, graduate, and post-doctoral fellows in molecular reproductive physiology, with a special emphasis on designing experiments, interpreting data, and superior presentation and publication of results.
- Provide trainees with the latest advancements in scientific investigation and methodologies in the field of reproductive biology.
- Facilitate a productive and collaborative interaction between science and implementation in the field.
- Contribute to the wellbeing of humans, animals, and the environment.
- Provide a creative, supportive, collaborative, challenging, and enjoyable environment for scientific research in the field of reproductive physiology, with a special emphasis on female reproductive function.
Extracellular Vesicle-coupled miRNAs in Stress Adaptation
Our lab is currently investigating the mechanisms underlying the packaging and release of precise miRNA cargo into EVs and their potential use to offset the detrimental effects of environmental stressors that ultimately affect granulosa cell function and oocyte/ embryonic development.
Oviductal Organoids in Assisted Reproductive Technologies
Organoids represent biomimetic, three-dimensional, in vitro cell clusters that organizationally self-regenerate into complex structures to mirror the physiological function of their tissue of origin. Our lab currently utilizes organoids to study and recapitulate oviductal physiology in vitro, with the potential to farm EVs for downstream use in current IVF systems.
Deciphering the impact of Advanced Maternal Age on Fertility
A focus in our lab is to understand the cellular and extracellular molecular responses of ovarian follicular cells and their corresponding EVs to advanced maternal age (AMA) and to investigate the possibility of developing strategies to tackle AMA-associated infertility problems.
Nrf2-mediated Oxidative Stress Response
In this project, we are aiming to investigate the impact of modulating Nrf2-Keap1 signaling in bovine embryos, primarily through pharmacological (antioxidant activation) and genetic (CRISPR-Cas9 mediated knockout of Keap1) approaches on subsequent embryo survival and viability under suboptimal conditions.
Antioxidant Roles in Follicular Cells and Oocytes
To improve the in vitro culture environment, our lab investigates the impact of various antioxidants (quercetin, carnosol, sulforaphane, etc.) to counteract the detrimental undertakings caused as a result of exposure to both oxidative and/or environmental stressors.
Bovine oviductal organoids: a multi-omics approach to capture the cellular and extracellular molecular response of the oviduct to heat stress
Menjivar NG, Gad A, Thompson RE, Meyers MA, Hollinshead FK, Tesfaye D. BMC Genomics 2023; 24(1):646.
Extracellular vesicle-microRNAs mediated response of bovine ovaries to seasonal environmental changes
Gad A, Joyce K, Menjivar NG, Heredia D, Rojas CS, Tesfaye D, Gonella-Diaza A. . J Ovarian Res. 2023 May 23;16(1):101.
Granulosa cell-derived extracellular vesicles mitigate the detrimental impact of thermal stress on bovine oocytes and embryos
Menjivar NG, Gad A, Gebremedhn S, Ghosh S, Tesfaye D. Frontiers in Cell and Developmental Biology; 11.
Dynamics of extracellular vesicle-coupled microRNAs in equine follicular fluid associated with follicle selection and ovulation
Gebremedhn S, Gad A, Ishak GM, Menjivar NG, Gastal MO, Feugang JM, Prochazka R, Tesfaye D, Gastal EL. Mol. Hum. Reprod. 2023.
Tesfaye D, Menjivar N, Gebremedhn S. Reprod. Fertil. Dev. 2021; 34(2):174–189.more publications
Funding: Colorado Center for Reproductive Medicine
Tesfaye Lab, Fall 2023
news and updates
Three Colorado State University students were recently recognized by the United States Department of Agriculture (USDA), National Institute of Food and Agriculture (NIFA), Agriculture and Food Research Initiative (AFRI) Predoctoral Fellowship Program, a leader as one of the largest federal funding agencies propelling cutting-edge discoveries that impact people’s daily lives and the nation’s future. The […]
Animal Reproduction and Biotechnology Laboratory Building, Foothills Campus
3105 Rampart Rd, Fort Collins, CO 80521