The Borlee laboratory, as part of the Center for Vector-Borne Infectious Diseases (CVID) and Center for Metabolism of Infectious Diseases(C4MInD) at Colorado State University, studies environmental sensing and regulation of bacterial behaviors in the context of host-pathogen interactions and host-associated bacterial communities. Biofilm formation is one such behavioral response that allows bacteria to survive stressful conditions in the environment and persist during chronic infections in humans. We study a variety of bacterial pathogens and endosymbionts of mosquitoes and midges. Our primary focus has been on Pseudomonas aeruginosa and Burkholderia pseudomallei as they are highly amenable to genetic, molecular, and developmental studies of biofilm formation and pathogenicity. The laboratory conducts research on the unanswered questions concerning the composition, function, and developmental control of biofilm matrix components and their role during infection of P. aeruginosa and B. pseudomallei. Some additional areas of research in the laboratory include c-di-GMP-mediated gene regulation, biofilm-associated disease, quorum sensing, antibiotic resistance, and development of strategies to prevent and treat infections (e.g. vaccines, drug therapies, and paratransgenic control of vector borne diseases).
Building biofilms to study the functions of molecules that are unique to biofilm metabolism.
Developing biofilm as visualized with confocal microscopy.
Develop paratransgenic approaches to interfere with viral replication
Goal: Characterize the microbiomes of arthropods (e.g. mosquitoes and midges) to develop paratransgenic approaches to alter vector competency.
Understand and interfere with biofilm-associated infections
Goal: Characterize the growth, regulation, and physiology of biofilms and devise strategies to disrupt biofilm-associated infections.
Defend against Burkholderia pseudomallei infections
Goal: Develop a vaccine to prevent Burkholderia pseudomallei infections in addition to therapeutics to rapidly treat melioidosis in patients that become infected.
Disruption of c-di-GMP Signaling Networks Unlocks Cryptic Expression of Secondary Metabolites during Biofilm Growth in Burkholderia pseudomallei.
Borlee GI, Mangalea MR, Martin KH, Plumley BA, Golon SJ, Borlee BR.
Appl Environ Microbiol. 2022 Mar 31:e0243121. doi: 10.1128/aem.02431-21. Online ahead of print. PMID: 35357191
Belardinelli JM, Li W, Martin KH, Zeiler MJ, Lian E, Avanzi C, Wiersma CJ, Nguyen TV, Angala B, de Moura VCN, Jones V, Borlee BR, Melander C, Jackson M.
Int J Mol Sci. 2022 Mar 9;23(6):2950. doi: 10.3390/ijms23062950. PMID: 35328372
The NarX-NarL two-component system regulates biofilm formation, natural product biosynthesis, and host-associated survival in Burkholderia pseudomallei.
Mangalea MR, Borlee BR.
Sci Rep. 2022 Jan 7;12(1):203. doi: 10.1038/s41598-021-04053-6. PMID: 34997073
Complete Genome Sequences of Eight Streptococcus equi subsp. zooepidemicus Strains Isolated from Mares in Estrus with Endometritis.
Borlee GI, Lakin SM, Kapuscinski ML, Abdo Z, Stenglein MD, McCue PM, Borlee BR.
Microbiol Resour Announc. 2021 Jul;10(26):e0132120. doi: 10.1128/MRA.01321-20. Epub 2021 Jul 1.
Identification of a PadR-type regulator essential for intracellular pathogenesis of Burkholderia pseudomallei.
McMillan IA, Norris MH, Zarzycki-Siek J, Heacock-Kang Y, Sun Z, Borlee BR, Hoang TT.
Sci Rep. 2021 May 17;11(1):10405. doi: 10.1038/s41598-021-89852-7. PMID: 34001967
news and updates
Two graduate students, mentored by Brad Borlee, received awards for their research posters at the 2017 CVMBS Research Day.
PhD student Mike Mangalea, mentored by Brad Borlee, wins CVMBS Top Scholar at the 2016 CSU Graduate Showcase.
Brad Borlee is named a 2013 Boettcher Webb-Waring Early Career Research Investigator awardee.
Office: Research Innovation Center (RIC) room D122
Lab: Research Innovation Center (RIC) room D143