The completion of the genome sequences of Mycobacterium tuberculosis, M. leprae and other mycobacterial species has led to a greater understanding of host-pathogen interactions of these intracellular human pathogens. Although humans are considered the main host of M. leprae, research in our laboratory has contributed to findings that M. leprae has adapted to other mammalian species including red squirrels in the British Isles, armadillos in Brazil and Mexico and recently wild chimpanzees in Guinea-Bissau and Ivory Coast, West Africa, leading to speculation that M. leprae is capable of adapting to other animal or environmental reservoirs. Dr. Spencer spends up to 2-3 months a year working in the laboratory of Dr. Claudio Salgado in Marituba, Pará, Brazil on leprosy research and field surveillance activities with the support of two Fulbright Scholar to Brazil awards in 2015-2016 and 2019-2020.
Our research is focused on the following:
- Understanding the humoral and cell-mediated immune responses to mycobacterial infection, mainly tuberculosis and leprosy.
- Identifying biomarkers of infection detected by anti-PGL-I serology, cytokine/chemokine assays, metabolomics or by molecular means (PCR, microRNA).
- Working with an international consortium of leprosy researchers in Brazil and the Netherlands to develop a simple lateral flow device that detects both antibody and cell-mediated responses to M. leprae antigens that can identify those asymptomatic individuals who are progressing towards disease.
- Identifying novel M. leprae strain types and drug resistance markers circulating in human clinical isolates and animal species performed by post-doctoral researcher Dr. Charlotte Avanzi.
The overall goals of the above projects are geared towards the development of new diagnostic tools capable of diagnosing leprosy early prior to the development of nerve damage, disfigurement and disability, and identifying other environmental or animal reservoirs of this pathogen.
Use of Immunological and Genetic Markers to Understand Infection and Transmission of Mycobacterium Leprae in Brazil
A consortium of leprosy researchers from CSU, Fiocruz Brazil, and the Federal University of Para, Brazil seek to identify immunological and genetic markers to understand leprosy infection and transmission in Brazil and develop new tools to increase the effectiveness of leprosy control programs targeting high-risk populations living in a hyperendemic area.
Hockings KJ, Mubemba B, Avanzi C, Pleh K, Düx A, Bersacola E, Bessa J, Ramon M, Metzger S, Patrono LV, Jaffe JE, Benjak A, Bonneaud C, Busso P, Couacy-Hymann E, Gado M, Gagneux S, Johnson RC, Kodio M, Lynton-Jenkins J, Morozova I, Mätz-Rensing K, Regalla A, Said AR, Schuenemann VJ, Sow SO, Spencer JS, Ulrich M, Zoubi H, Cole ST, Wittig RM, Calvignac-Spencer S, Leendertz FH.
Nature. 2021 Oct;598(7882):652-656. doi: 10.1038/s41586-021-03968-4. Epub 2021 Oct 13.
Latent leprosy infection identified by dual RLEP and anti-PGL-I positivity: Implications for new control strategies.
PLoS One. 2021 May 13;16(5):e0251631. doi: 10.1371/journal.pone.0251631. eCollection 2021.PMID: 33984058
PLoS Negl Trop Dis. 2020 Dec 10;14(12):e0008917. doi: 10.1371/journal.pntd.0008917. eCollection 2020 Dec.PMID: 33301536
Sci Rep. 2020 Jul 28;10(1):12648. doi: 10.1038/s41598-020-69355-7.PMID: 32724108
Front Microbiol. 2020 May 11;11:711. doi: 10.3389/fmicb.2020.00711. eCollection 2020.PMID: 32477280
news and updates
Identified in chimpanzees, leprosy joins a growing list of pathogens circulating in the environment that afflict both humans and other animals.
Alum John Spencer has pursued an exciting career focused on leprosy, around the world and in the lab, since graduating from Avon Old Farms.
The first confirmed cases of leprosy affecting wild chimpanzees, suggests an unidentified reservoir in the environment.
Office: Microbiology room B208
Lab: Microbiology room C210